Antibody and Immunoassay Services, HKU
Antibody and Immunoassay Services, HKU

 

Type:
Recombinant
Tag:
His
Source:
E.Coli
Species:
Mouse
Other names:
FGF21
Cat. No.:
42189
Size:
0.1 mg
Purity
>95%
Files:

Introduction to the Molecule

FGF21, a polypeptide with 210 amino acid residues produced mostly from the liver tissue.[1] Mouse FGF21 shares 75% identity as human FGF21. Recent animal studies indicate it possesses potent beneficial effects on glucose and lipid metabolism and insulin sensitivity.[2] Increasing data shows FGF21 can significantly stimulate glucose uptake in mature adipocytes. And The lowered LDL-cholesterol and increased HDL-cholesterol can also be observed.[2,3] FGF21 exerts its bioactivity through interaction with membrane bound FGF receptors (FGFRs) which requires β-Klotho as a co-factor to bind and activate FGFR signaling.[4,5]The activation of FGF21 can induce the stimulation of diverse downstream pathways medicated by MAPK,FRS-2, SHP-2, PI3K, raf, stat and other signaling molecules.[6-9] In sum, FGF21 induces a variety of significant beneficial metabolic changes without apparent adverse effects which makes this factor a hot candidate to treat some metabolic diseases.[10]

Amino Acid Sequence

GA AY PIPDSSPLLQ FGGQVRQRYL YTDDDQDTEA HLEIREDGTV VGAAHRSPES LLELKALKPG VIQILGVKAS RFLCQQPDGA LYGSPHFDPE ACSFRELLLE DGYNVYQSEA HGLPLRLPQK DSPNQDATSW GPVRFLPMPG LLHEPQDQAG FLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

Formulation

Filtered (0.22 μm) and lyophilized in 1 mg/mL in PBS

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1  mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage.

Quality Control Test

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.

Applications

Cell culture and/or animal studies, ELISA, Western blotting, Immunoprecipitation,

Reference

[1] Nishimura T, Nakatake Y, Konishi M, Itoh N: Identification of a novel FGF, FGF-21, preferentially expressed in the liver. Biochim Biophys Acta1492:203–206, 2000
[2] Kharitonenkov A et al. FGF-21 as a novel metabolic regulator. J Clin Invest 115:1627–1635, 2005
[3]Alexei Kharitonenkov et al. The Metabolic State of Diabetic Monkeys Is Regulated by Fibroblast Growth Factor-21.Endocrinology .148(2):774–781. 2007
[4] Hiroshi Kurosu et al. Tissue-specific Expression of βKlotho and Fibroblast Growth Factor (FGF) Receptor Isoforms Determines Metabolic Activity of FGF19 and FGF21. J Biol Chem. 282(37): 26687–26695. 2007
[5] Ogawas Y et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21 Proc Natl Acad Sci USA 104: 7432-7437, 2007.
[6]Steven L. PELECH et al. Fibroblast growth factor treatment of Swiss 3T3 cells activates a
subunit S6 kinase that phosphorylates a synthetic peptide substrate. Proc. Natl. Acad. Sci. USA
Vol. 83, pp. 5968-5972, August 1986
[7]Rosa Carballada et al. Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathway during Xenopus mesoderm induction. Development 128, 35-44 (2001)
[8]Dayanand D. Deo et al. Phosphorylation of STAT-3 in Response to Basic Fibroblast Growth
Factor Occurs through a Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical Vein Endothelial Cells. The journal of molecular chemistry. Vol. 277, No. 24, Issue of June 14, pp. 21237–21245, 2002
[9]Maria I. Kontaridis et al. Role of SHP-2 in Fibroblast Growth Factor Receptor-Mediated
Suppression of Myogenesis in C2C12 Myoblasts. Molecular and cellular biology, Vol. 22, No. 11, p. 3875–3891. 2002
[10] Dostálová I, Haluzíková D, Haluzík M.Fibroblast Growth Factor 21: A Novel Metabolic Regulator With Potential Therapeutic Properties in Obesity/Type 2 Diabetes Mellitus. Physiol. Res. 58: 1-7, 2009

Representative publications cited AIS mouse FGF21 protein

[1] Adiponectin mediates the metabolic effects of FGF21 on glucose homeostasis and insulin sensitivity in mice.Lin Z, Tian H, Lam KS, Lin S, Hoo RC, Konishi M, Itoh N, Wang Y, Bornstein SR, Xu A, Li X. Cell Metab. 2013 May 7;17(5):779-89.

[2] Fibroblast growth factor 21 induces glucose transporter-1 expression through activation of the serum response factor/Ets-like protein-1 in adipocytes.Ge X, Chen C, Hui X, Wang Y, Lam KS, Xu A.J Biol Chem. 2011 Oct 7;286(40):34533-41.

 

 


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