Filtered (0.22 μm) and lyophilized in 1 mg/mL in PBS
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Cell culture and/or animal studies, ELISA, Western blotting, Immunoprecipitation,
 Nishimura T, Nakatake Y, Konishi M, Itoh N: Identification of a novel FGF, FGF-21, preferentially expressed in the liver. Biochim Biophys Acta1492:203–206, 2000
 Kharitonenkov A et al. FGF-21 as a novel metabolic regulator. J Clin Invest 115:1627–1635, 2005
Alexei Kharitonenkov et al. The Metabolic State of Diabetic Monkeys Is Regulated by Fibroblast Growth Factor-21.Endocrinology .148(2):774–781. 2007
 Hiroshi Kurosu et al. Tissue-specific Expression of βKlotho and Fibroblast Growth Factor (FGF) Receptor Isoforms Determines Metabolic Activity of FGF19 and FGF21. J Biol Chem. 282(37): 26687–26695. 2007
 Ogawas Y et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21 Proc Natl Acad Sci USA 104: 7432-7437, 2007.
Steven L. PELECH et al. Fibroblast growth factor treatment of Swiss 3T3 cells activates a
subunit S6 kinase that phosphorylates a synthetic peptide substrate. Proc. Natl. Acad. Sci. USA
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Rosa Carballada et al. Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathway during Xenopus mesoderm induction. Development 128, 35-44 (2001)
Dayanand D. Deo et al. Phosphorylation of STAT-3 in Response to Basic Fibroblast Growth
Factor Occurs through a Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical Vein Endothelial Cells. The journal of molecular chemistry. Vol. 277, No. 24, Issue of June 14, pp. 21237–21245, 2002
Maria I. Kontaridis et al. Role of SHP-2 in Fibroblast Growth Factor Receptor-Mediated
Suppression of Myogenesis in C2C12 Myoblasts. Molecular and cellular biology, Vol. 22, No. 11, p. 3875–3891. 2002
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Representative publications cited AIS mouse FGF21 protein
 Adiponectin mediates the metabolic effects of FGF21 on glucose homeostasis and insulin sensitivity in mice.Lin Z, Tian H, Lam KS, Lin S, Hoo RC, Konishi M, Itoh N, Wang Y, Bornstein SR, Xu A, Li X. Cell Metab. 2013 May 7;17(5):779-89.
 Fibroblast growth factor 21 induces glucose transporter-1 expression through activation of the serum response factor/Ets-like protein-1 in adipocytes.Ge X, Chen C, Hui X, Wang Y, Lam KS, Xu A.J Biol Chem. 2011 Oct 7;286(40):34533-41.