Antibody and Immunoassay Services, HKU
Antibody and Immunoassay Services, HKU

 

Polyclonal Antibody Development

We provide quality services for production and purification of polyclonal antibodies in New Zealand rabbits. Our standard immunization protocol is summarized below, which can be modified depending on the immunogenecity of different antigens. We also provide technical support for antigen preparation (expression and purification of recombinant proteins, design and conjugation of peptide antigens etc), purification of antibodies using the affinity chromatography, and antibody characterization etc.

Days

Task

Notes

Day 1

1st Immunization

100ug antigen per rabbit

Day 21

2nd Immunization

100ug antigen per rabbit

Day 42

3rd Immunization

100ug antigen per rabbit

Day 49

Bleed test

ELISA to evaluate titer

Day 56

Bleed production

At least 40ml serum per rabbit


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