Recombinant full-length human FGF-21 expressed in E.Coli.
The antibody detects circular human FGF-21.
Lyophilized in 1 mg/mL in PBS
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Store at –20°C. For long-term storage, aliquot and freeze at -70°C.
Avoid repeated freeze/defrost cycles.
ELISA - This antibody can be used at 2?g/mL with the appropriate secondary reagents to detect human FGF-21.
Western blot, Immunoprecipitation and immunocytochemistry are not tested.
Fibroblast growth factor 21(FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic ?-cells[3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance.
 Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635
 Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437
 Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta;1492: 203– 206
 Kurosu H, Choi M, et al. (2007)J Biol Chem; 282: 26687– 26695
 Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35.
 Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
 Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.