Antibody and Immunoassay Services, HKU
Antibody and Immunoassay Services, HKU

 

FGF-21 (human), rabbit polyclonal antibody

Polyclonal Antibody against Human FGF-21

Host:
Rabbit
Cat. No.:
11180-01
Size:
0.1 mg
Files:

Isotype/Preparation:

Rabbit specific IgG was purified by human FGF21 affinity chromatography

Immunogen:

Recombinant full-length  human FGF-21 expressed in E.Coli.

Specificity:

The antibody detects circular human FGF-21.

Formulation:

Lyophilized in 1 mg/mL in PBS

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1  mg/mL and let the lyophilized pellet dissolve completely.

Storage:

Store at –20°C. For long-term storage, aliquot and freeze at -70°C.
Avoid repeated freeze/defrost cycles.

Application/Usage:

ELISA - This antibody can be used at 2μg/mL with the appropriate secondary reagents to detect human FGF-21.
Western blot, Immunoprecipitation and immunocytochemistry are not tested.

Introduction

Fibroblast growth factor 21(FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic β-cells[3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents[5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon[6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance[7].

Reference:

[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 16271635
[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426437
[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta;1492: 203206
[4] Kurosu H, Choi M, et al. (2007)J Biol Chem; 282: 2668726695
[5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35.
[6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
[7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.

 

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