Antibody and Immunoassay Services, HKU
Antibody and Immunoassay Services, HKU

 

Appl1 (human) , rabbit polyclonal

Affinity Purified Polyclonal Antibody against Human APPL1

Host:

Rabbit

Cat. No.:

11130

Size:

0.1 mg

Files:

Isotype/Preparation:

Rabbit antiserum was purified by affinity APPL1 coupled colum

Immunogen:

Recombinant full-length human APPL1 expressed in E. coli cells

Specificity:

The antibody detects many types of APPL1 in different species such as human, monkey, mouse, rat etc. (about 85kDa)

Formulation:

Solution in PBS. Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Application/Usage:

Western blot - This antibody can be used at 0.1 - 0.2 µg/mL with the appropriate secondary reagents to detect APPL1.
Immunostaining - This antibody can be used at 1.0 -2.0 µg/mL with the appropriate secondary reagents to detect APPL1.
ELISA - This antibody can be used at 0.5 - 1.0 µg/mL with the appropriate secondary reagents to detect APPL1.
Immunoprecipitation – See reference [6], [11]

Introduction

APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain [1], was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait [2]. APPL1 binds to a number of cell surface receptors (TrkA[3, 4], DCC[5], adiponectin [6, 7], FSH[8]) and intracellular signaling molecules (small GTPase Rab5[9], GIPC[4] and inositol 5- phosphatase[10], suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3 [11].

Reference:
1. Hosch, S.E., J.M. Olefsky, and J.J. Kim, APPLied mechanics: uncovering how adiponectin modulates insulin action. Cell Metab, 2006. 4(1): p. 5-6.
2. Mitsuuchi, Y., et al., Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2. Oncogene., 1999. 18(35): p. 4891-8.
3. Lin, D.C., et al., APPL1 associates with TrkA and GIPC1, and is required for NGF-mediated signal transduction. Mol Cell Biol, 2006. 25: p. 25.
4. Varsano, T., et al., GIPC is recruited by APPL to peripheral TrkA endosomes and regulates TrkA trafficking and signaling. Mol Cell Biol, 2006. 26(23): p. 8942-52.
5. Liu, J., et al., Mediation of the DCC apoptotic signal by DIP13 alpha. J Biol Chem., 2002. 277(29): p. 26281-5. Epub 2002 May 14.
6. Cheng, K.K., et al., Adiponectin-induced endothelial nitric oxide synthase activation and nitric oxide production are mediated by APPL1 in endothelial cells. Diabetes, 2007. 56(5): p. 1387-94.
7. Mao, X., et al., APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol., 2006. 8(5): p. 516-23. Epub 2006 Apr 16.
8. Nechamen, C.A., et al., Human follicle-stimulating hormone (FSH) receptor interacts with the adaptor protein APPL1 in HEK 293 cells: potential involvement of the PI3K pathway in FSH signaling. Biol Reprod., 2004. 71(2): p. 629-36. Epub 2004 Apr 7.
9. Miaczynska, M., et al., APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment. Cell., 2004. 116(3): p. 445-56.
10. Erdmann, K.S., et al., A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway. Dev Cell, 2007. 13(3): p. 377-90.
11. Cheng, K.K., et al., APPL1 potentiates insulin-mediated inhibition of hepatic glucose production and alleviates diabetes via Akt activation in mice. Cell Metab, 2009. 9(5): p. 417-27.

 

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