Antibody and Immunoassay Services, HKU
Antibody and Immunoassay Services, HKU

 

Human adiponectin, rapid

Rapid Human Adiponectin ELISA Kit (Cat. No. 31012)

Sample type:
serum, plasma,adipocyte extract and cell culture supernatant
Sample requirement:
0.1ul / well
Calibration range:
1.55~100 ng/ml
Size:
96 wells (1 kit)
Files:

ASSAY CHARACTERISTICS

A. Sensitivity

    The lowest level of human adiponectin that can be detected by this assay is 1.55 ng/ml.

B. Specificity:

Analyte
Cross Reactivity
 
Mouse adiponectin
No
 
Rat adiponectin
No
 
Human resistin
No
 
Human TNFa
No
 
Human insulin
No
 
Human leptin
No
 
Human IL6
No
 
Human ANGPL4
No
 

C. Precision:

Intra-assay Precision

Sample

Mean (ng/ml)

SD (ng/ml)

CV (%)

1

9.21

0.37

4.02

2

21.33

0.78

3.65

Inter-assay Precision

Sample

Mean (ng/ml)

SD (ng/ml)

CV (%)

3

5.32

0.24

4.53

4

15.72

0.79

5.01

D. Spiking Recovery:

      97%~105%

Representative publications cited AIS human Adiponectin ELISA kit

[1] Adiponectin mediates the metabolic effects of FGF21 on glucose homeostasis and insulin sensitivity in mice.Lin Z, Tian H, Lam KS, Lin S, Hoo RC, Konishi M, Itoh N, Wang Y, Bornstein SR, Xu A, Li X. Cell Metab. 2013 May 7;17(5):779-89.

[2] Elevated circulating adipocyte-fatty acid binding protein levels predict incident cardiovascular events in a community-based cohort: a 12-year prospective study. Chow WS, Tso AW, Xu A, Yuen MM, Fong CH, Lam TH, Lo SV, Tse HF, Woo YC, Yeung CY, Cheung BM, Lam KS. J Am Heart Assoc. 2013 Jan 15

[3] High serum level of fibroblast growth factor 21 is an independent predictor of non-alcoholic fatty liver disease: A 3-year prospective study in China. Li H, Dong K, Fang Q, Hou X, Zhou M, Bao Y, Xiang K, Xu A, Jia W. J Hepatol. 2012 Nov 7.

[4] Combined use of serum adiponectin and tumor necrosis factor-alpha receptor 2 levels was comparable to 2-hour post-load glucose in diabetes prediction. Woo YC, Tso AW, Xu A, Law LS, Fong CH, Lam TH, Lo SV, Wat NM, Cheung BM, Lam KS. PLoS One. 2012;7(5)

[5] Adipocyte Fatty Acid Binding Protein levels relate to inflammation and fibrosis in Non Alcoholic Fatty Liver Disease. 6. Milner KL, Poorten D, Xu A, Bugianesi E, James G. Kench JG, Lam KSL, Chisholm. DJ. Jacob George J. Hepatology, 2009 Jun;49(6):1926-34

[6] Adiponectin isoform distribution in women--relationship to female sex steroids and insulin sensitivity. Leung KC, Xu A, Craig ME, Martin A, Lam KS, O'Sullivan AJ. Metabolism. 2009 Feb;58(2):239-45.

[7] Circulating levels of adipocyte and epidermal fatty acid-binding proteins in relation to nephropathy staging and macrovascular complications in type 2 diabetic patients.Yeung DC, Xu A, Tso AW, Chow WS, Wat NM, Fong CH, Tam S, Sham PC, Lam KS. Diabetes Care. 2009 Jan;32(1):132-4.

[8] Hypoadiponectinemia is related to hypoxemia and sympathetic activation in sleep obstructive aponea. Lam JC, Xu A, Tam S, Khong PI, Yao TJ, Lam DC, Lai AY, Lam B, Lam KS, Mary SM. Sleep. 2008 Dec 1;31(12):1721-7.

[9] Epidermal fatty-acid-binding protein: a new circulating biomarker associated with cardio-metabolic risk factors and carotid atherosclerosis. Yeung DC, Wang Y, Xu A, Cheung SC, Wat NM, Fong DY, Fong CH, Chau MT, Sham PC, Lam KS. Eur Heart J. 2008 Sep;29(17):2156-63

[10] Adiponectin mediates the suppressive effect of rosiglitazone on plasminogen activator inhibitor-1 production. Hoo RL, Chow WS, Yau MH, Xu A, Tso AW, Tse HF, Fong CH, Tam S, Chan L, Lam KS. Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2777-82.

[11] Serum adiponectin is increased in advancing liver fibrosis and declines with reduction in fibrosis in chronic hepatitis B. Hui CK, Zhang HY, Lee NP, Chan W, Yueng YH, Leung KW, Lu L, Leung N, Lo CM, Fan ST, Luk JM, Xu A, Lam KS, Kwong YL, Lau GK; for The Hong Kong Liver Fibrosis Study Group. J Hepatol. 2007 Aug;47(2):191-202.

[12] Hypoadiponectinemia as a predictor for the development of hypertension: a 5-year prospective study. Chow WS, Cheung BM, Tso AW, Xu A, Wat NM, Fong CH, Ong LH, Tam S, Tan KC, Janus ED, Lam TH, Lam KS. Hypertension. 2007 Jun;49(6):1455-61.

[13] Circulating adipocyte-fatty acid binding protein levels predict the development of the metabolic syndrome: a 5-year prospective study. Xu A, Tso AW, Cheung BM, Wang Y, Wat NM, Fong CH, Yeung DC, Janus ED, Sham PC, Lam KS. Circulation. 2007 Mar 27;115(12):1537-43.

[14] Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes. Xu A, Chan KW, Hoo RL, Wang Y, Tan KC, Zhang J, Chen B, Lam MC, Tse C, Cooper GJ, Lam KS. J Biol Chem. 2005 May 6;280(18):18073-80.

 

 


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